Synthesis and Characterization of Electrospun Sorbent for the Solid-Phase Extraction of Fluoroquinolones in Human Plasma and Their UHPLC-PDA Determination

In this work we investigated the synthesis and characterization of electrospun polyacrylonitrile (PAN) polymethyl methacrylate (PMMA) stabilized in air, made a 5:1 ratio, used as sorbent for solid-phase extraction fluoroquinolones plasma samples following quantification UHPLC-PDA. Preliminary analyses viscosity were carried out on polymer solution to be sure about electrospinability. Characterizations performed membrane evaluate morphology (SEM scanning electron microscopy AFM atomic force microscopy), thermal degradation behavior (TGA thermogravimetric analysis), porosity surface area (BET, Brunauer Emmett Teller), quantitative qualitative distribution structures (FTIR infrared analysis Fourier transform EDX Energy Dispersive X-ray analysis). A method was developed by studying parameters such amount pH sample. Finally, UHPLC-PDA validated accordance with guidelines successfully applied. The use prepared combined has allowed development whose strengths are its speed, accuracy, sensitivity, high recoveries.